The Difference in Blast Number Between Manual Count And ADVIA 2120i Automatic Hematology Analyzer
Keywords:Blood smear evaluation, Blast numbers, Leukemia, ADVIA 2120i
INTRODUCTION. The development of sophisticated automated blood-cell analyzers caused the proportion of blood-count samples that require a manual different count has steadily diminished. Blood smear remains a crucial diagnostic aid in determining type of leukemia by the appearance and blast numbers in blood smear. ADVIA 2120i has a parameter of blast cells percentage. This study was undertaken to determine the difference in blast number between manual count and ADVIA 2120i automatic hematology analyzer.
METHOD. This was an analytical observational study with cross-sectional design. Thirty samples (22 peripheral blood and 8 bone marrow) which were detected blast numbers from ADVIA 2120i were examined. Samples were collected from November 2015 to August 2016. Manual count was performed in each sample by using blood smear and bone marrow evaluation.
RESULTS. Twenty three cases of AML and 7 cases of ALL were found. Blast percentage from manual count was between 0 to 95% (Mean 28.5%); from Advia 2120i was between 0.1 to 99% (Mean 16.2%). There was significant difference in conformity results from blast number between manual count and ADVIA 2120i with p < 0.05.
DISCUSSION. The difference of blast numbers between manual count and ADVIA 2120i could be caused by: 1) in manual count, determining blast cells is based on cytoplasm characteristics, granules, nuclear cells, nuclear chromatin, nucleoli. 2) in ADVIA 2120i, determining blast cell is based on complexity and resistancy from BASO reagent.
CONCLUSION. There were significant differences in blast numbers between manual count and ADVIA 2120i
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